Fortgeschrittene Modul 'Molecular Protein Engineering
Contact: Dr. Bert van Loo, b.vanloo[at]uni-muenster.de, room 100.22, tel: 21636
The course takes place in block I (April/May) of the 'Sommer Semester', starting on the first Monday of the respective block. The total length of the course is 4 weeks. The first two weeks consist of lectures, practicals and some exercises, which are all intermixed to accommodate the planning of the experimental work carried out during the practical. During the first two weeks we start at 10.00 (sharp) at Lab 10 of Schlossplatz 4, although some lectures and exercises will be at the Institute for Evolution and Biodiversity in the afternoon (details to be announced during the course). The last two weeks there are no lectures or practicals and you will have time to work on the 'protein engineering challenge' case study and prepare for the exam. The exam will be on the 2nd of May 2018 at 14.00-16.00h in the social room of the IEB. The presentations for the 'protein engineering challenge' case study will be on Friday the 11th of May 2018, 13.00-16.00h, also in the social room of the IEB.
This course is an introduction into the principles of molecular protein engineering techniques and their application in biotechnological processes. Techniques that will be covered include various mutagenesis techniques directed at a specific site within a protein, as well as more random approaches such as Directed Evolution, and strategies developed based on historical evolution such as Ancestral Sequence Reconstruction.
The course will comprise lectures on the general principles of molecular protein engineering (mutagenesis techniques, screening strategies etc..) in the first two weeks.
The practicals will cover a single round of directed evolution of a single enzyme and will cover at least one of the mutagenesis techniques covered in the lectures. There will also be two in silico practicals dealing with molecular docking and ancestral sequence reconstruction.
The third part of the course is a 'case study' in which each student will be given a 'protein engineering challenge'. This encompasses the design of a research strategy to achieve the goal described in the challenge using a molecular protein engineering-based approach. The topics for the case studies will be chosen/assigned at the latest at the start of the second week.